diff --git a/pkgs/development/python-modules/macholib/default.nix b/pkgs/development/python-modules/macholib/default.nix
new file mode 100644
index 000000000000..f58bcfe18057
--- /dev/null
+++ b/pkgs/development/python-modules/macholib/default.nix
@@ -0,0 +1,55 @@
+{
+  lib,
+  buildPythonPackage,
+  fetchFromGitHub,
+  pythonOlder,
+  unittestCheckHook,
+  altgraph,
+  setuptools,
+  typing-extensions,
+  pyinstaller,
+}:
+
+buildPythonPackage rec {
+  pname = "macholib";
+  version = "1.16.3";
+  pyproject = true;
+
+  src = fetchFromGitHub {
+    owner = "ronaldoussoren";
+    repo = pname;
+    rev = "v${version}";
+    hash = "sha256-bTql10Ceny4fBCxnEWz1m1wi03EWMDW9u99IQiWYbnY=";
+  };
+
+  build-system = [ setuptools ];
+
+  dependencies =
+    [
+      altgraph
+    ]
+    ++ lib.optionals (pythonOlder "3.11") [
+      typing-extensions
+    ];
+
+  nativeCheckInputs = [
+    unittestCheckHook
+  ];
+
+  passthru.tests = {
+    inherit pyinstaller; # Requires macholib for darwin
+  };
+
+  preCheck = ''
+    export PATH="$PATH:$out/bin"
+  '';
+
+  meta = with lib; {
+    description = "Analyze and edit Mach-O headers, the executable format used by Mac OS X.";
+    homepage = "https://github.com/ronaldoussoren/macholib";
+    changelog = "https://github.com/ronaldoussoren/macholib/releases/tag/v${version}";
+    license = licenses.mit;
+    platforms = platforms.darwin;
+    maintainers = with maintainers; [ eveeifyeve ];
+  };
+}
diff --git a/pkgs/top-level/python-packages.nix b/pkgs/top-level/python-packages.nix
index 5f4c18055d16..09fbd6fd81de 100644
--- a/pkgs/top-level/python-packages.nix
+++ b/pkgs/top-level/python-packages.nix
@@ -7509,6 +7509,8 @@ self: super: with self; {
     inherit (pkgs.darwin.apple_sdk.frameworks) CoreFoundation CoreServices;
   };
 
+  macholib = callPackage ../development/python-modules/macholib { };
+
   macropy = callPackage ../development/python-modules/macropy { };
 
   maec = callPackage ../development/python-modules/maec { };